Paraoxonase Activity In Type 2 Diabetes Mellitus Patients With And Without Complications
Published: February 1, 2011 | DOI: https://doi.org/10.7860/JCDR/2011/.1166
RENUKA SUVARNA*, SOUMYA S RAO*, CHITRALEKHA JOSHI*, VIVEKANANDA KEDAGE*, MANJUNATHA S MUTTIGI*, JEEVAN K SHETTY**, MUNGLI PRAKASH***
*MSC, Department of Biochemistry, Kasturba Medical College, Manipal, India; **MD, Department of Biochemistry, Kasturba Medical College, Manipal, India; ***MD, Department of Biochemistry and Genetics, St Matthew’s University, School of medicine, Grand, Cayman, Cayman Islands, BWI
Correspondence
Dr. Prakash Mungli MD.,
Department of biochemistry and genetics,
St Matthew's University, School of Medicine,
P.O.BOX 30992, Regatta Office Park, Leeward Three,
Grand Cayman KY1-1204, CAYMAN ISLANDS, BWI.
Tel: +1 345 814 3187
Email: prakashmungli@yahoo.co.in
Introduction: Type 2 diabetes mellitus (DM) is a disease of metabolic dysregulation. The current study was undertaken to understand the relationship between the fasting lipid profile and the paraoxonase 1 (PON1) activity in type 2 diabetic patients, with and without complications.
Materials and methods: The study group consisted of a total of 155 subjects, which included non-diabetic healthy control subjects (n = 50) and diabetic patients with complications (group I, n = 66) and without complications (group II, n=39). PON1 activity was measured in all the subjects, based on spectrophotometric methods and the fasting lipid profile and the fasting plasma glucose (FPG) levels were determined by using a clinical chemistry analyzer, Hitachi 912.
Results: FPG (p<0.001) and triacylglycerides (TAG) (p<0.001) were significantly increased and high-density lipoprotein-cholesterol (HDL-C) (p<0.001) and PON1 (p<0.001) were significantly decreased in group I patients as compared to the normal controls. In group II patients, FPG (p<0.001), TAG (p<0.001) and total cholesterol (TC) (p<0.05) were significantly increased and HDL-C (p<0.05) was significantly decreased as compared to the normal controls. By using Pearson’s correlation, HDL-C was found to be positively correlated with PON1 in group I patients (r=0.317, p<0.01).
Conclusion: Type 2 DM patients with complications have significantly decreased HDL-C levels and PON1 activity, possibly indicating their decreased biochemical roles in these patients.
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